How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription

Classical models of gene expression were built using genetics and biochemistry. Although these approaches are powerful, they have very limited consideration the spatial temporal organization expression. dynamics RNA polymerase II (RNAPII) transcription machinery fundamental functional consequences for expression, its detailed studies been abrogated by limits classical light microscopy a long time. The advent super-resolution (SRM) techniques allowed visualization RNAPII with nanometer resolution millisecond precision. In this review, we summarize recent methodological advances in SRM, focus on application nanoscale space time transcription, discuss mechanistic understanding